We further interrogated the potential clinical utility of routine (that is, ADA assay) and new same-day diagnostic tools (that is, uIFNγ and Xpert MTB/RIF) by comparing positive (LR+) and negative (LR-) likelihood ratios (Table 2) and positive (PPV) and negative (NPV) predictive values at different prevalence rates of TB (TB prevalence = 30% in Table 2, TB prevalence of 10%, 30%, and 50% presented in Additional file 1: Table S5). The gene discussed is ADA; the disease is tuberculosis.