Similarly, the ERK1/2 phosphorylation was fully supressed only in cells with increased EGFR GCN and WT KRAS. In conclusion, this study indicates that the diagnostic algorithm combining EGFR IHC and EGFR SISH is a highly promising method for selecting CRC patients benefiting from anti-EGFR treatment. Here, EGFR is linked to colorectal carcinoma.