Signal pathway analysis showed the vitamin D-upregulated pathways are mainly involved in fatty acid, bile acid, vitamin A and drug metabolic pathways, whereas the vitamin D-downregulated pathways include those responsible for mTOR signaling pathway, glycosaminoglycan biosynthesis, adhesion, tyrosine, vitamin A and fatty acid metabolic pathways (Table 1b).These genes or pathways might be used as targets for finding novel drug for cholangiocarcinoma treatment, and be used in clinical diagnosis in the future studies. The gene discussed is MTOR; the disease is cholangiocarcinoma.