To further verify LCN2 role in human ICC, we examined 80 human ICC specimen and 66% of human ICC specimen presented with high expression LCN2, adding the finding that knockdown of LCN2 in SNU308 cells decreased cell growth and migration (Fig 4f), indicating the LCN2 plays as an oncogene in human ICC. The gene discussed is LCN2; the disease is intrahepatic cholangiocarcinoma.