INS and type 2 diabetes mellitus: We used fatty acid overload as condition for inducing lipotoxicity as it is unambiguously a condition which is present in insulin resistant and T2DM patients (Fraze et al. 1985; Chen et al. 1987; Golay et al. 1987; Swislocki et al. 1987; Reaven et al. 1998), and found that all cellular and molecular parameters studied in this paper are impaired in accordance with that seen in vivo conditions.