The nested PCR was used to further verify the candidate genes in the peripheral blood samples of the 43 breast cancer patients and 20 healthy control subjects, whereby three marker genes, including FAM83A (NM_032899.4), NPY1R (NM_000909.5) and KRT19 (NM_002276), were identified as the novel panel of markers. This evidence concerns the gene NPY1R and breast cancer.