The aim of the present study was to determine whether colon cancer cells, which overexpressed FBXL20, showed signs of an abnormal Wnt signaling pathway by measurement of the β-catenin, E-cadherin, SET, p53, caspase 3, PP2A, c-Myc and Axin expression levels, in addition, cell proliferation and migration ability were observed. The gene discussed is FBXL20; the disease is colonic neoplasm.