In order to study PEMT involvement in tumor growth and progression concurrently with other lipid-related enzymes associated with tumor progression, such as FASN and LPL, FASN activity ratios (ratio of activity in cancer tissue to that in adjacent non-cancer tissue for each individual patient) and LPL activity ratios (ratio of activity in cancer tissue to that in adjacent non-cancer tissue for each individual patient) were used, which were estimated previously in the same tissues and shown to predict shorter patient survival (18–20). This evidence concerns the gene FASN and cancer.