In order to study PEMT involvement in tumor growth and progression concurrently with other lipid-related enzymes associated with tumor progression, such as FASN and LPL, FASN activity ratios (ratio of activity in cancer tissue to that in adjacent non-cancer tissue for each individual patient) and LPL activity ratios (ratio of activity in cancer tissue to that in adjacent non-cancer tissue for each individual patient) were used, which were estimated previously in the same tissues and shown to predict shorter patient survival (18–20). Here, PEMT is linked to cancer.