CTLA4 and type 1 diabetes mellitus: We then examined serum at 1/20 and 1/40 dilutions from individuals affected with T1D and GD compared with age- and sex-matched healthy controls using the sCTLA-4 as well as the pan–CTLA-4 assay (Fig. 6C for representative data; Supplemental Fig. 3A–D shows raw data for all samples).