HMGB1 and steatosis: Ethanol-fed Hmgb1fl/fl−/−Alb-Cre− mice showed enhanced HMGB1 expression and translocation (Fig. 5C) and developed significant liver injury and steatosis as shown macroscopically and by H&E staining, serum alanine aminotransferase, serum and liver triglycerides, and activity scores (steatosis, inflammation, and necrosis), whereas Hmgb1fl/flAlb-Cre mice were protected from ALD (Fig. 5D).