Considering the marked up-regulation of the Vegfa transcript in response to OPNc overexpression in both OvCar-3 and PC-3 cells, and also previous data from our group demonstrating that conditioned medium (CM) secreted from cells overexpressing OPNc (OPNc-CM) is able to stimulate most OPNc tumor-causing features [6,8], we used this CM to further validate part of these array data. The gene discussed is VEGFA; the disease is neoplasm.