BAX and infection: Following DENV challenge at 0.1 MOI with each serotype, 1×106 transformed C6/36 clonal cells stably expressing αDENV-U143-ΔN Bax constructs were washed and stained with FITC-conjugated annexin V at 48 hours post infection and analyzed on a 96 well microtiter plate as described in Methods (Figure 8A).