To elucidate the functional role of FIP200 on HMGB1 translocation, we transfected MH-S cells with a myc-FIP200 plasmid and observed that FIP200 overexpression facilitated the cytosol translocation of HMGB1 from nuclei upon PAO1 infection (Fig. 4A). The gene discussed is HMGB1; the disease is infection.