Transfection of GFP-USP26 into HEK293 cells revealed nuclear localization with an accumulation and colocalization with AR in subnuclear foci which is similar to the effect of a specific AR mutation of the DNA-binding domain which confers androgen insensitivity syndrome (AIS) in patients via impaired ligand-dependent nuclear translocation, subnuclear foci formation, and intranuclear mobility of the receptor[19], [49], [50]. The gene discussed is AR; the disease is androgen insensitivity syndrome.