RT-qPCR assays for TMED3 and SOX12 with normal colon and colon cancer patient samples of different TNM stages plus liver metastases obtained from the operating room (Supplementary Fig S8; Varnat et al, 2010) confirmed that TMED3 mRNA was consistently expressed at higher levels in tumors over normal colon, or over normal liver in the case of liver metastases (Supplementary Fig S6). This evidence concerns the gene TMED3 and malignant colon neoplasm.