YAP1 and neoplasm: Red/green assays with shYAP1-GFP+ CC14 cells injected subcutaneously into nude mice revealed that YAP1 is cell autonomously essential for tumor growth as green cells disappeared from the tumor (Fig 4E and F, Supplementary Fig S4), again showing the opposite phenotype of shTMED3- and shSOX12-expressing cells (Fig 4B and D).