Analysis of the expression of a canonical WNT-TCF signaling signature in CC14 colon cancer cells by RT-qPCR after expression of either shTMED3 or shSOX12 showed that both induced a drastic reduction in TCF target gene expression levels, largely paralleling the effects of TCF blockade with dnTCF4 (Fig 8A). Here, HNF4A is linked to colonic neoplasm.