Since downregulation of WNT-TCF targets has been linked to increased HH-GLI activity in colon cancer (Varnat et al, 2009), we tested for the expression of key readouts of activity and mediators of this pathway (GLI1, GLI2) as well as the expression of the GLI-interacting stemness factor NANOG (Zbinden et al, 2010) and three other members of a HH-GLI-regulated embryonic stem cell-like signature: SOX2, KLF4 and OCT4 (Clement et al, 2007; Varnat et al, 2010). This evidence concerns the gene GLI2 and malignant colon neoplasm.