Reduced GLO1 activity in the Glo1KDApoe−/− mice increased MG‐H1 immunoreactivity in extracts of aorta (Fig. 6A and B) and kidney (not shown); however, the Glo1KDApoe−/−mice did not develop more atherosclerosis at 22 weeks of age in either the aortic root (Fig. 6C, E and F) or the aortic arch (Fig. 6D) and actually showed a trend toward decreased lesion area in the aortic root (mean average lesion area calculated from the four transverse sections, Glo1KDApoe−/−: 0.038 ± 0.008, Apoe−/−: 0.071 ± 0.013, P = 0.06). This evidence concerns the gene APOE and atherosclerosis.