A representative IgG response to Stx2 detected by WB in sera of HUS patients and control subjects is shown in Figure 1B. It is worth mentioning that considering the child’s age, the frequency of reactivity either to A subunit or both subunits of Stx2 was considerably higher in HUS children less than 2 and 2–3 years old compared to controls in the same age range (Figure 2A and B). The gene discussed is STX2; the disease is hemolytic-uremic syndrome.