ESR1 and hepatocellular carcinoma: Consistent with MTT data, butein treatment for 24 hours caused cleavages of PARP and Caspases (Caspase-8, −9 and −3) in T47D (luminal, ER+), HCC-70 (basal A, TNBC), BT-474 (luminal, ER+/PR+/HER2+) and HCC-1569 (basal A, HER2+), while not affecting those in luminal HER2+ HCC-1419, HCC-2218 and SKBR-3 cells (Figure 2A).