Likewise, butein at 10 μg/ml for 24 hours increased Annexin V-positive and PI-positive populations in T47D, HCC-70, BT-474 and HCC-1569 cells; whereas it did not affect HCC-1419, HCC-2218 and SKBR-3 cells (Figure 2B). This evidence concerns the gene ANXA5 and hepatocellular carcinoma.