The results showed that stressed mice developed exacerbated NASH compared to wild-type mice as judged by increased levels of serum ALT and AST (t (13) = 3.271, p = 0.006; t (11) = 2.735, p = 0.0194), and NAFLD activity inflammation scores (t (18) = 2.742, p = 0.0134; t (18) = 3.899, p = 0.001) (Figure 5).To figure out which cytokines take part in NASH, we detected the cytokines closely related to NASH in both liver and serum. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.