Although the stimulated let-7a expression by the ectopic HMGA2 overexpression was not entirely proofed within this study the newly generated CT1258-EGFP-HMGA2 cell line is nevertheless a suitable tool to further investigate the impact of HMGA2 expression on other HMGA2 regulating and regulated genes in canine prostate cancer. This evidence concerns the gene HMGA2 and Familial prostate cancer.