It was found that GnT-V act as a good prognostic factor which has lower expression in human lung cancer than in normal lung, and GnT-V expression was negative correlated with EMT mesenchymal markers, we also found that GnT-V expression and its products β1,6-GlcNAc modification of cell surface were altered during TGF-β1-induced EMT in lung cancer cells. The gene discussed is MGAT5; the disease is lung carcinoma.