Hence, inhibition of β1,6-GlcNAc branched N-glycans' formation, shRNA-mediated knockdown of GnT-V and overexpression of wild-type GnT-V or inactivated GnT-V were employed to specifically examine the role of GnT-V in TGF-β1-induced EMT, cell migration and invasion in lung cancer cells. The gene discussed is TGFB1; the disease is lung cancer.