Here, we report the functional characterization of hematopoietic progenitor kinase 1 (HPK1)-interacting protein of 55 kDa (HIP-55)/Drebrin-like (DBNL), which was identified in a 14-3-3γ affinity resin-based proteomic approach using lung adenocarcinoma A549 cells. Here, MAP4K1 is linked to lung adenocarcinoma.