The greatest and least prevalence of toxoplasmosis belongs to Rey city and shiraz, respectively (13, 14).Overall, this infection is common in mild and dry foothills of west of Iran with 33-68 percent prevalence rate (15).There are two methods for toxoplasmosis detection, CLIA and ELSA (15).Quantity of IgG and IgM change during infection, therefore, CLIA and ELSA methods can help to recognize toxoplasmosis, these methods are cheap, quick and highly repeatable. This evidence concerns the gene CD40LG and infection.