In the present study, we treated 3xTg-AD mice, a commonly used transgenic model of AD which harbors three mutated transgenes (human PS1M146V, APPSWE, and tauP301L), with propofol, a commonly used intravenous anesthetic in clinical practice, and investigated the effects of intranasal insulin on propofol-induced hyperphosphorylation of tau. The gene discussed is INS; the disease is Alzheimer disease.