HA-1077 reduced the level of reactivity with anti-ROCK-I and anti-ROCK-II antibodies to very weak staining in 20-30% of tumor cells, while the extent of staining for anti-RhoA antibody was not changed much, but its intensity was reduced to weak or moderate.Western blotting revealed that LPA and GGOH increased RhoA activity, as well as the expression of ROCK-I and ROCK-II (Figure 3). The gene discussed is ROCK2; the disease is neoplasm.