MARCHF7 and neuroblastoma: To study which axotrophin sequences contribute to the different subcellular localizations, we transiently expressed different EGFP-tagged axotrophin domains: N-terminal domains (axoN1 aa 1–625, axoN2 1–541, axoN3 1–344) and C-terminal domains (axoC1 aa 333–704, axoC2 542–704) in N2A mouse neuroblastoma cells (Fig. 6) and in COS-7 cells (data not shown).