This result indicates that insulin successfully activated the intracellular insulin signaling pathway in the isolated mature adipocytes.Interestingly, total Akt protein and its insulin-responsive phosphorylation were both greater in mature adipocytes isolated from HFD-IFNT mice than HFD-Control mice (Figure 3C).Taken together, our results suggest that IFNT treatment effectively modulates obesity-associated insulin resistance at least by suppressing tissue inflammation. Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.