In particular, Prx-I, the markedly down-expressed protein after treatment, is directly linked to transcription factor NF-kappa-B (NF-κB) complex pathway in this network, indicating that Prx-I may play an important role in the apoptosis of bladder cancer by BI-TK/GCV system partly through the NF-κB signaling pathway. The gene discussed is NFKB1; the disease is urinary bladder cancer.