Actually, based on the iTRAQ analysis (Table S1), Western blot (Fig. 3A) and IHC analysis (Fig. 3B) in the present study, Prx-I expression significantly decreased in bladder cancer tissues after treatment with BI-TK/GCV, indicating that Prx-I may contribute to the effect of BI-TK/GCV treatment on anti-growth and pro-apoptosis of bladder cancer. This evidence concerns the gene PRDX1 and urinary bladder cancer.