AKT1 and infection: To determine whether the down-regulation of Akt-mTOR signaling observed in FAK−/− cells affects Salmonella survival, WT and FAK−/− PEMs were incubated with S. typhimurium for 30 minutes, treated with gentamycin to kill extracellular bacteria, and intracellular bacterial survival was quantified after a further 1, 2.5 and 4.5 hours of infection.