MRC-5 cells were infected with HSV-1 in the present of Hsp90 inhibitors (0.8 μM), and total RNA samples were extracted at 4, 6 and 9h post-infection (p.i.), and viral UL54 (Immediate early gene), UL29 (Early gene) and UL27 (Late gene) were assayed by quantitative real-time PCR, respectively (Fig. 1A). This evidence concerns the gene RPL35 and infection.