This data was confirmed by calcium imaging technique, which showed how TRPV1, even if present, did not respond to capsaicin (alone or in combination with capsazepine) activation at noncytotoxic concentrations in all cancer cell lines, whereas a significant calcium influx was described, in positive controls, after ionomycin (nonselective cation channel agonist) administration. The gene discussed is TRPV1; the disease is cancer.