Cells infected with the complemented ΔespG mutant (ΔespG + pEspG:HA) exhibited reduced Tf-binding at an earlier time-point than WT EHEC (from 2.5 h post infection), however by 10 h post infection both WT EHEC and EHEC ΔespG + pEspG:HA retained less than 50% of the amount of Tf on the cell surface as uninfected cells or cells infected with EHEC ΔespG mutant. Here, TF is linked to infection.