Twist1 is implicated in the differentiation of multiple cell lineages including muscle formation, and regulates maturation and differentiation of human ovarian cancer stem cells through induction of the aforementioned miRNAs and subsequent inhibition of Pten and Ikkβ by those miRNAs that lead to inhibition of NF-κB and activation of Akt pathways [77]. This evidence concerns the gene PTEN and ovarian cancer.