EGFR and lung carcinoma: Because the SmartAmp2 method is a simple and reliable method to detect mutations, it is often utilized in clinical settings, for example, for highly sensitive detection of the EGFR mutation in lung cancer [18], rapid and highly sensitive detection of the 2009 pandemic influenza A (H1N1) virus [19], and simple detection of multiple polymorphisms of the β3AR, β2AR, and UCP1 genes in many subjects [10].