The present study aimed to describe the profile of E. coli from Mexican women with urinary tract infection by the identification of virulence genes (fimH, papC, sfa/focDE, sfaS, focG, ecpA, ecpR-B, hlyA, cnf-1, cdt-B, cvaC, iutA, ibeA, rfc, tratT, and fyuA), phylogenetic group, and their resistance to antibiotics to guide better diagnosis and treatment of UTI. This evidence concerns the gene PCDH8 and urinary tract infection.