In the present study, we plan to study whether (1) ROS are involved in insulin-regulated glycolysis in hepatocellular carcinoma cells; (2) insulin regulates PKM2 expression via ROS production; (3) insulin upregulates PKM2 expression in ROS dependent manner through miRNAs expression; and (4) PKM2 is required for insulin-induced aerobic glycolysis. The gene discussed is INS; the disease is hepatocellular carcinoma.