FAS and breast cancer: Furthermore, the inhibition of FAS could also induce changes in the synthesis of membrane phospholipids and subsequently assembling of lipid rafts, as well impair the proper localization of EGFR to the cell membrane in breast cancer cells[35].Considering the main function of FAS is catalyzing the biosynthesis of long chain fatty acid, we determined the intracellular free fatty acid by Free Fatty Acid Quantification Kit.