To understand how ACSVL3 contributes to the phenotype of GBM neurosphere cells, we generated ACSVL3 knockdown GBM neurosphere cells by transiently transfecting the cells with two ACSVL3 siRNAs (si3 and si4) that target different regions of ACSVL3 mRNA. Here, SLC27A3 is linked to glioblastoma.