GPC3 and hepatocellular carcinoma: Immunohistochemical analysis was positive for CK7, CK20 (weak), CK18, CDX2 (weak), Hep Par-1, TTF1; and negative for GPC3, AFP, and napsin A. Hence, a diagnosis was made of HCC with metastases to the lung, bone, brain, kidney, adrenal gland, subcutaneous tissues, and abdominal pelvis.