GPC3 and hepatocellular carcinoma: Immunohistochemical analysis was positive for KI-67 (60%), CK20 (interspersed), CK, villin (focal), Hep Par-1, mammaglobin, CDX2, and TTF1; and negative for vimentin, GPC3, GCDFP-15, CK5/6, and napsin A. This confirmed the histological diagnosis of HCC.