Our approach was to focus on the allergic asthma counter-regulatory IFNγ gene promoter, susceptible to epigenetic regulation following environmental exposures[16], and due to the more sensitive approach of pyrosequencing we identified cell/tissue specific methylation differences in a gene not previously reported by Stefanowicz et al. The strong correlations between CpGs -186 and -54 in CD4+ lymphocytes and weaker correlations between the same sites in buccal cells reported here also highlight the varying behavior of neighboring CpG methylation in different tissues. This evidence concerns the gene CD4 and allergic asthma.