While ANGPTL4 was upregulated by TGF-β in both MDA-MB-231 and M3 cells in vitro, we found that ANGPTL4 was actually downregulated by TGF-β/Smad3 in vivo in the M3 tumors where TGF-β functions as a tumor suppressor. The gene discussed is SMAD3; the disease is neoplasm.