Before STZ injection, HFD-fed rats had similar glucose concentrations to NPD-fed rats, but significantly higher serum insulin, triglyceride, total cholesterol, and free fatty acids concentrations, Injection of STZ (25 mg kg-1), The level of blood glucose significantly increased compared to HFD-fed rats and NPD-fed rats, thus suggesting producing type 2 diabetes with insulin resistance. The gene discussed is INS; the disease is type 2 diabetes mellitus.