ERBB2 and breast carcinoma: An anchorage-dependent 3D cell culture model utilizing lrECM as a scaffold, an anchorage-independent model utilizing poly-HEMA to induce spheroid formation and a 2D monolayer cell culture model were employed to test a library of 102 drugs and compounds against the JIMT1 breast cancer cell line (over-expresses Her2) in a 384-well microtitre plate format [76].