In this study, we validated the polymerase chain reaction (PCR)-based high resolution melt (HRM) assay for screening DNMT3A, IDH1 and IDH2 mutations in samples obtained from patients with AML at diagnosis and developed 2 rapid methods for detecting more common mutations, DNMT3A R882H and IDH2 R140Q. Here, DNMT3A is linked to acute myeloid leukemia.