We next analyzed IL-27 expression in PBMC culture supernatants and observed that unstimulated PBMCs from patients with active BD produced a lower level of IL-27 (585.7 ± 218.7 pg/ml) than unstimulated PBMCs from patients with inactive BD (1,041.2 ± 193.2 pg/ml; P = 0.021) and unstimulated PBMCs from healthy controls (1,103.5 ± 232.9 pg/ml; P = 0009). Here, IL27 is linked to Behcet disease.