We found that hyperglycemia induced a large downshift in the diaphragm contractile protein force-pCa relationship, reducing Fmax from 182.9 ± 1.85 kPa to 85.7 ± 1.99 kPa (this is an index of actin-myosin crossbridge formation) (P <0.001) and decreasing the coefficient of cooperativity, N, from 5.98 to 5.17 (P = 0.015). Here, MYH14 is linked to Hyperglycemia.