To assess the performance of our method, low numbers (i.e., 5, 13, or 25 cells) of various non-small cell lung tumor H1975, A549 or H1755 cells expressing varying levels of EpCAM were spiked into 4 mL of normal blood, and processed according to our protocol for tumor cell isolation with the On-chip Sort system. Here, EPCAM is linked to neoplasm.