As shown in Figure 4C, the mRNA expression of IRS-4 was not consistently affected in the drug-induced cell lines used in this study.The mRNA expression of IGF-1 and IGF-2 is consistently increased in all the cancer cell lines compared to their respective parental cells except MCF-7 cell line which did not show a significant increase in IGF-1 (Figure 5). Here, IGF1 is linked to cancer.