BRAF and neoplasm: In the discrepant cases, we observed 20 (37%) patients with a wild-type first tumor and a mutated subsequent tumor, 14 (26%) with a mutated first tumor and a wild-type subsequent tumor, 8 (15%) with change in alteration variants between the two tumor lesions, and 12 (22%) with an additional gene amplification in the two BRAF-mutated tumors (3 cases in first but not in subsequent tumors and 9 with an opposite condition).