In order to investigate the relationship between MELK and chemoresistance to 5FU in gastric cancer cells, SGC7901 and NCI-N87 cells were exposed to different concentrations of 5-FU (0, 1, 2, 4, 8, 16 μg/ml), and cells were then collected after 48 h for qPCR analysis and Western blotting to determine the MELK expression level. Here, MELK is linked to gastric cancer.