E, enncapsulation of DTX in nanoparticles could induce moreincreased apoptosis inof prostate cancer cells through the activation of the caspase-2 pathway[41].Given that SPM demonstrated stronger in vitro cytotoxicity than free DTX, we performed apoptosis assays using Annexin V-FITC and PI staining to compare apoptosis induction by SPM and Duopafei. This evidence concerns the gene ANXA5 and prostate carcinoma.